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Structure and mechanism of the complex between cytochrome P4503A4 and ritonavir

The reduction in BW from baseline was 22% at the maximum dose tested (mean SEM, 100 nmol/kg from 43.6 1.6 g to 34.8 1.4 g) and 10% from baseline at the lowest dose tested (mean SEM, 1 nmol/kg, from 42.5 1.3 g to 38.2 1.3 g) (Figure 1, A and B)

Lactate Dehydrogenase (LDH) Assay The relative amounts of LDH released into the media (conducted in triplicates for each group) were determined using CytoTox-ONE Homogeneous Membrane Integrity Assay kit Cat.#G7892 (Promega, USA) according to the manufacturers instructions